rapid detection methods for foodborne pathogens
Feature extraction from light-scatter patterns of Listeria colonies for identification and classification. We have isolated several enterohemorrhagic E. coli (EHEC) strains from naturally contaminated ground beef (an ongoing project from Alabama A&M University). 2011. Microfabricated device for impedance-based detection of bacterial metabolism. PARTICIPANTS: Nothing significant to report during this reporting period. 2013, 2014, 2019a, b). Food Technol. Food Control Journal of Biomedical Optics 13(1): 014010. Biosensors and Bioelectronics. Innate reverse transcriptase activity of DNA polymerase for isothermal RNA direct detection. Fiber optic sensor was developed for detection of the three major pathogens using specific biorecognition molecules. Applied Optics. 2007. This system monitors interaction of live pathogens or active toxins with live mammalian cells and highly efficient for detection of low amounts of harmful target analytes. Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics. Development of a fiber-optic waveguide biosensor assay for the detection of Salmonella Enteritidis from poultry products. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Bi, L., Yang, L., Bhunia, A.K. Detection of foodborne pathogens at an early stage is very important to control food quality and improve medical response. One micro liter supernatant was used as target for colorimetric assay, specificity and anti-interference experiments. 2006. Huang, T., T. Geng, D. Akin, W.J. Proceeding of SPIE. The cytotoxicity patterns were comparable to those with EHEC obtained from various outbreaks. Institute of Food Technologist Annual Meeting, Chicago, IL, July 28-Aig 1, 2007, Abstr. This system was also demonstrated to be useful for identification of bacterial colonies belonging to genus, Salmonella, Escherichia, Huff, K. 2008. APMIS Suppl. Microbiol. Figure 1. Bhunia. Amass, S., A. K. Bhunia, A. Chaturvedi, D. Dolk, S. Peeta and M. Atallah 2006. This format will be developed to test for Salmonella surface antigens. Hence, a variety of methods have been developed for rapid detection of P-04, p490. (IME)-chip was employed to measure Listeria interaction with Ped-2E9 cells using an impedance analyzer. Gut Pathog. In this work, we utilized RNA one-step detection of denaturation bubble-mediated Strand Exchange Amplification (SEA) method to realize the detection of viable foodborne pathogen S. aureus. Label - free detection of bacterial pathogens using light scattering sensor from food. RewriteRule ^index.php$ - [L] Surface plasmon resonance immunosensor can detect soluble L. monocytogenes antigens in 15 minutes but is unable to detect intact cells.Publications, Progress 10/01/00 to 09/30/01OutputsOne of our goals is to develop methods that would be able to detect very low numbers of Listeria monocytogenes rapidly from food. Medina, S. 2011. Status: McGraw-Hill Professional, New York, NY. Antibodies are critical for immunosensor applications. ImpactsWhat was accomplished under these goals? Right click on the X and choose Properties. (4) In the past year we have initiated a project on detection of Listeria by using a surface plasmon resonance immunosensor (IAsys sensor). 2006. Front Microbiol. Journal of Applied Microbiology 100:1017-1027. RewriteCond %{REQUEST_FILENAME} !-d NON-TECHNICAL SUMMARY: With the increase in reports of foodborne infections, great attention has been given to the development of new/improved methods for rapid detecting microbial pathogens. 109:808-817. Tu, S-I., Geng, T., Uknalis, J., and Bhunia, A.K. Williams, L.L. 2010. 2012. Chem. Light scattering, fiber optic and cell-based sensor for sensitive detection of foodborne pathogens. Published Detection and identification of foodborne pathogens in genus and species levels using a noninvasive modified light scatterometer, BARDOT. Singh, N., R.K. Singh, A.K. P-099, p499. This antibody was used in a resonant mirror immunosensor and the antibody was able to detect surface protein extracts from L. monocytogenes and L. innocua and showed no reaction with other Listeria species. A., Hnsch, G. M., Filler, S. G., et al. 2011. S. aureus, Staphylococcus aureus; SEA, strand exchange amplification; POCT, point-of-care testing; PCR, polymerase chain reaction; ELISA, enzyme linked immunosorbent assay; NASBA, nucleic acid sequence-based amplification; LAMP, loop-mediated isothermal amplification; SDS, sodium dodecyl sulfate; HPLC, high performance liquid chromatography; NTC, no target control; PAGE, polyacrylamide gel electrophoresis. 2013 Bioengineered Probiotics-A Solution to Broaden Probiotics Efficacy! Food Sci Biotechnol. P3-49. 2006. 2007. Ariefdjohan, M.W., P.E. 2005. WebThe conventional methods used to detect foodborne pathogen are time consuming and laborious. 2012. Bzikot, J. Zawistowski, and A.K. Detection of Listeria monocytogenes using an automated fiber optic biosensor: Raptor. Jaradat, and A.K. In the Abstracts of the USEPA Microbial Source Tracking Workshop. [5] Genomic fingerprinting of E. coli O157:H7: Preliminary results indicated that genomic fingerprint patterns generated by AFLP and Rep-PCR could effectively Simon. Hirleman, and A.K. Paris, France: ACM. Bhunia. The fluorescence signal of the SEA reaction was detected by the Gentier 48S Isothermal amplification fluorescence detection system at 1 min intervals. Kim, H., Bai, N., Bhunia, A.K., King, G.B., Hirleman, E.D., and Bae, E. 2013.
Phase 2: Characterization and Screening of the Libraries - Once libraries are generated, they will be used for selection of recombinant monoclonal antibodies against various surface antigens of Salmonella, by immobilizing the antigen of interest and applying various selection rounds with the appropriate phage display libraries. Applied and Environmental Microbiology 74 (15): 4853-4866. Label-free detection of Escherichia coli O157:H7 and Listeria monocytogenes from food samples using light-scattering sensor. doi: 10.1099/mic.0.083485-0, Shi, C., Shang, F., Zhou, M., Zhang, P., Wang, Y., and Ma, C. (2016). (2011). P-041, p445. On platforms that enforce case-sensitivity PNG and png are not the same locations. 2005. The advent of biotechnology has introduced new technologies that led to the emergence of rapid diagnostic methods and altered food testing practices. Mira Miralles M, Maestre-Carballa L, Lluesma-Gomez M, Martinez-Garcia M. Foods. Future challenges to microbial food safety. A rapid and simple SEA method for the detection of S. aureus was developed in this work. Hand-held biosensor for rapid detection of hemolytic and cytolytic toxins in foods and beverages. Gut Pathogens 5:16. Glucose and nutrient concentrations affect the expression of a 104-kDa Listeria adhesion protein (LAP) in Listeria monocytogenes. Journal of Rapid Methods and Automation in Microbiology. SEA detection kits were obtained from Qingdao Navid Biotechnology Co. Ltd. (China). 2003. The SEA reaction was performed according to the manufacturers' instructions under optimal conditions. Type: 70: 6138-6146. You can try renaming that file to .htaccess-backup and refreshing the site to see if that resolves the issue. Ladisch. and Yao, Y. sharing sensitive information, make sure youre on a federal In support of multipathogen detection, a multipathogen enrichment broth, SEL, was formulated. (Di Febo, 2019) 4. To evaluate the sensitivity of SEA detection method for S. aureus (Zhang et al., 2018), different concentrations of DNA fragments and genomic DNA of S. aureus were detected. Bacteria-mediated delivery of nanoparticles into cells. This varies by browser, if you do not see a box on your page with a red X try right clicking on the page, then select View Page Info, and goto the Media Tab. Published 2010. American Society for Microbiology General Meeting, Salt Lake City, UT. Antibodies and immunoassays for detection of bacterial pathogens. PCR method is J. Agric. Hybridoma Ped-2E9 cells cultured under modified conditions can sensitively detect Listeria monocytogenes and Bacillus cereus. The present review discusses the reasons for the increasing interest in rapid methods, current developments in the field, the research needs, and the future trends. da Silva, J.A. J. Infect. Crit Rev Food Sci Nutr. WebAn accuracy of 98% was achieved when the rapid assay was applied to measure the recovery of inoculated samples. The laser based light scattering sensor can detect specific bacterial pathogens while growing on an agar plate based on their scattering properties without the need for any biorecognition molecules. 2. 77, 366371. Graduateand undergraduate students, postdoctoral scientists, and visiting international scientistis and scholars received training in this project. HHS Vulnerability Disclosure, Help Naschansky, K.N., S. Budiarty, A. Sharma, M. Morgan, R. Bashir, and A.K. Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii. 2001. 2009. Published Kim, M.T. Kim, K.P., T. Geng, and A. K. Bhunia. Rapid label free sero-diagnosis of Johnes disease using surface plasmon resonance biosensor. About 76% of those isolates showed 0-45% Vero cell toxicity by lactate dehydrogenase (LDH) assay and 24% exhibited 45-83% toxicity. 2016 Jul 3;56(9):1568-84. doi: 10.1080/10408398.2013.782483. Cambridge University, Manchester, UK. Burkholder, K.M., Kim, K.-P., Hahm, B.-K. Mishra, K., Medina-Maldonado, S., and Bhunia, A.K. P-058. FoodMicro2008. Chem. Journal Articles Sirsat, S.A., Burkholder, K.M., Dowd, S.E., Muthaiyan, A., Bhunia, A.K., and Ricke, S.C. 2011. 2023 Feb 12;8(1):77. doi: 10.3390/biomimetics8010077. Status: N terminal Gly224-Gly411 Domain in Listeria adhesion protein (LAP) interacts with receptor Hsp60. Rajwa, B., Bayraktar, B., Banada, P.P., Huff, K., Bae, E., Hirleman, E.D., Bhunia, A.K., Robinson, J.P. 2008. May 21-25, 2006, Abstr. June 15-19, 2002. During this reporting period, we optimized mammalian cell-based sensor and light scattering sensors for their ability to detect and identify multiple pathogens including Salmonella, E. coli, and Listeria, and various toxins from food products. [2] Interdigitated microsensor electrode (IME)-chip based cytotoxicity assay: Mammalian cells (Ped-2E9) exposed to L. monocytogenes for 1-2 h was placed on the IME-chip, and the Ped-2E9 damage was measured by an Impedance Analyzer. and Bhunia, A.K. Redirects and rewriting URLs are two very common directives found in a .htaccess file, and many scripts such as WordPress, Drupal, Joomla and Magento add directives to the .htaccess so those scripts can function. Banerjee, P., M.T. P-91, p575. Chapter 25; pp 414-421. As shown in Figure 3, the SEA method could realize the detection of viable S. aureus by one-step detection of RNA by the fluorescence signal. Biotechnology and Bioengineering 92(6):685-693. Rev. Despite 2010. Book Chapters There are general standard methods for determination of the foodborne pathogens and mycotoxins in food products including conventional methods such as culture and microscopic methods, chemical and biological methods (immunological, molecular genetic methods, gel diffusion), there are also more rapid methods which Biomimetic Material for Quantification of Methotrexate Using Sensor Based on Molecularly Imprinted Polypyrrole Film and MWCNT/GCE. 2005. A cell-based sensor that employs collagen-trapped mammalian cells for onsite detection of Listeria and Bacillus toxins is shown to be promising and the results could be obtained in 4-6 h with inoculated food samples. Bae, E., Aroonnual, A., Bhunia, A.K., and Hirleman, E.D. Yang, L., P.P. In addition, Bst DNA polymerase has intrinsic reverse transcriptase activity (Shi et al., 2015), which makes the SEA method able to detect RNA directly without additional reverse transcription. 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