nucleoside diphosphate kinase mechanism

However, the regulatory mechanism of NDPK is poorly understood. Islam, K.; Burns, R.G. Vron M, Tepper A, Hildebrandt M, Lascu I, Lacombe ML, Janin J, Morra S, Cherfils J, Dumas C, Chiadmi M. Adv Exp Med Biol. Clipboard, Search History, and several other advanced features are temporarily unavailable. Mesnildrey, S.; Agou, F.; Karlsson, A.; Bonne, D.D. (1971). Nucleoside diphosphate kinase (NDPK; EC 2.7.4.6) catalyzes the transfer of terminal phosphate groups from 5-triphosphate to 5-diphosphate nucleotides. J. Biol. Introduction. . Crystals of the enzymes from Dictyostelium discoideum and from Drosophila melanogaster were treated with phosphoramidate, and their X-ray structures were determined at 2.1 and 2.2 A resolution, Other groups such as sugars and single-carbon groups can be substituted in this reaction instead of the phosphoryl group. This work has analyzed the kinetics parameters of several mutant NDP kinases modified on residues interacting with the gamma-phosphate and/or the 3'-OH of the Mg2+-ATP substrate and compared the relative contributions of the active-site residues and the substrate 3'- OH for point mutations on Lys16, Tyr56 and Asn119. Burgers, P. M., and Eckstein, F. (1978). 353, 85-92. ; Krissinel, E.B. Morelli MB, Marinelli O, Aguzzi C, Zeppa L, Nabissi M, Amantini C, Tomassoni D, Maggi F, Santoni M, Santoni G. Cancers (Basel). Congr. Methods Enzymol. 182, 319-326. In Techniques in Protein Chemistry (D.R. ). Crystal structure and biophysical characterization of the nucleoside diphosphate kinase from. AMP is metabolized to adenosine by ecto-5'-nucleotidase (NT5E/CD73) which is the dominant adenosine-generating . most exciting work published in the various research areas of the journal. Its axial ligands are a histidine nitrogen and a beta-phosphate oxygen. EMBO J. Xu, Y. W. (1998). Federal government websites often end in .gov or .mil. ; Kaur, P.; Sharma, S.; Singh, T.P. Although nucleotide analogues used in antiviral therapies are also metabolized to their triphosphate form by NDP kinase, their lack of the 3-hydroxyl of the ribose, which allows them to be DNA chain terminators, severely impairs the catalytic efficiency of NDP kinase. ; Georgescauld, F.; Lascu, I.; Dautant, A. Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis. ; Gardberg, A.S.; Edwards, T.E. Cherfils, J., Morera, S., Lascu, I., Veron, M., and Janin, J. Deville-Bonne, D., Sellam, O., Merola, F., Lascu, I., Desmadril, M., and Veron, M. (1996). NMP kinases are homologous proteins that have a conserved NTP-binding domain. A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach. The plant nucleoside diphosphate kinase (NDPK, EC 2.7.4.6) gene family consists of three groups whose gene products are found in different subcellular locations. Chem. Polosina, Y., Jarrell, K. F., Fedorov, O. V., and Kostyukova, A. S. (1998). 2020, 21, 6779. Advan. Epub 2022 Mar 8. We have examined the modification of NDPK in oxidative stresses. ; Omary, M.B. Lombardi, D.; Sacchi, A.; DAgostino, G.; Tibursi, G. The association of the Nm23-M1 protein and beta-tubulin correlates with cell differentiation. In this review we discuss advances in the structure, folding and stability of NDPKs. Diplme d'Etudes Approfondies, University of Bordeaux-2. Wolfe, K.; Kofuji, S.; Yoshino, H.; Sasaki, M.; Okumura, K.; Sasaki, A.T. EC number. This page was last edited on 7 September 2022, at 12:35. Chatterjee A, Eshwaran R, Poschet G, Lomada S, Halawa M, Wilhelm K, Schmidt M, Hammes HP, Wieland T, Feng Y. Structure, Folding and Stability of Nucleoside Diphosphate Kinases. Intern. Nucleoside diphosphate kinase reversibly transfers the gamma-phosphate of ATP onto its active site histidine. The interfaces between these oligomeric states are very different but, surprisingly, the assembly structure barely affects the catalytic efficiency of the enzyme. 6, 413-419. [11][12] Nucleoside monophosphate binding induces further changes that render the enzyme catalytically capable of facilitating a transfer of the phosphoryl group from ATP to nucleoside monophosphate.[13]. ; Altshuler, P.J. Struct. In both enzymes, the active site histidine was phosphorylated on N delta, and it was the only site of phosphorylation. The structure of the, Arai, S.; Yonezawa, Y.; Okazaki, N.; Matsumoto, F.; Tamada, T.; Tokunaga, H.; Ishibashi, M.; Blaber, M.; Tokunaga, M.; Kuroki, R. A structural mechanism for dimeric to tetrameric oligomer conversion in, Baugh, L.; Gallagher, L.A.; Patrapuvich, R.; Clifton, M.C. ; Schopf, J.W. those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). This cross-linking was reversible in response to . 45, 273-387. ; Dautant, A. NME1 (NME/NM23 Nucleoside Diphosphate Kinase 1) is a Protein Coding gene. AlF3 forms a trigonal bipyramid that makes it an accurate analog of the transition state of the gamma-phosphate of ATP undergoing transfer to the catalytic histidine. (Fig. Georgescauld, F.; Moyni, L.; Habersetzer, J.; Cervoni, L.; Mocan, I.; Borza, T.; Harris, P.; Dautant, A.; Lascu, I. Intersubunit ionic interactions stabilize the nucleoside diphosphate kinase of. Adenylate kinase (AK) is an enzyme that regulates adenine nucleotide metabolism which is widely present in higher organisms and bacteria, catalyzing the reaction ATP + AMP 2ADP. 1996 Nov 15;242(2):165-71. doi: 10.1006/abio.1996.0449. ; Liotta, L.A.; Sobel, M.E. Sikarwar, J.; Singh, P.K. Matte, A., Tavi, L. W., and Delbaere, T. J. Structure Rescue of the neuroblastoma mutant of the human nucleoside diphosphate kinase A/nm23-H1 by the natural osmolyte trimethylamine-N-oxide. ; Mathews, C.K. Anal. 93, 4009-4016. ; Mathews, C.K. sharing sensitive information, make sure youre on a federal Biochem. This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. Biochemistry ; Singh, Y. Cytotoxic activity of nucleoside diphosphate kinase secreted from, Ganaie, A.A.; Lella, R.K.; Solanki, R.; Sharma, C. Thermostable hexameric form of Eis (Rv2416c) protein of. -, Anal Biochem. Biochemistry doi: 10.1002/jcla.24367. (1972a). J. Biol. PubMedGoogle Scholar, Lascu, I., Gonin, P. The Catalytic Mechanism of Nucleoside Diphosphate Kinases. Lascu, I., Pop, R. D., Porumb, H., Presecan, E., and Proinov, I. Other groups such as sugars and single-carbon groups can be substituted in this reaction instead of the phosphoryl group. This is a preview of subscription content, access via your institution. The mitochondrial nucleoside diphosphate kinase (NDPK-D/NME4), a moonlighting protein for cell homeostasis. However, due to the lack of structural information concerning the interaction between NDPK and tubulin, the existence of a NDPKtubulin complex has been controversial in the field up to very recently [. MC159 protein from the molluscum contagiosum instead inhibits NF-kappa-B activation by interacting with the IkB kinase complex . ; Ishikawa, T.; Yang, P. General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase. 1996-2023 MDPI (Basel, Switzerland) unless otherwise stated. Magnesium or complexes of NTP's are the true substrates for this reaction. (1993). J. Mol. Alberty, W. J., and Knowles, J. R. (1976). This binding domain also consists of a sheet flanked by helices. Crystal structure of a nucleoside diphosphate kinase from. Modulation of cytoskeletal dynamics by mammalian nucleoside diphosphate kinase (NDPK) proteins. Shibue, R.; Sasamoto, T.; Shimada, M.; Zhang, B.; Yamagishi, A.; Akanuma, S. Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins. Carotenuto, M.; Pedone, E.; Diana, D.; de Antonellis, P.; Dzeroski, S.; Marino, N.; Navas, L.; Di Dato, V.; Scoppettuolo, M.N. Sun, J.; Singh, V.; Lau, A.; Stokes, R.W. The aim is to provide a snapshot of some of the FEBS Lett. ; Wawrzak, Z.; Onopriyenko, O.; Edwards, A.; Savchenko, A.; Anderson, W.F. NME7 is a functional component of the gamma-tubulin ring complex. 29, 401-416. Arch. The goal of this series is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. Hendricks, S. P., and Mathews, C. K. (1997) J. Biol. Ndks form hexamers or two types of tetramers made of the same building block, namely, the common dimer. White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.) Yellow-Leaf 1 encodes a magnesium-protoporphyrin IX monomethyl ester cyclase. Pedersen, P. L., and Cattarall, W. A. 2), ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase1/CD39) hydrolyzes ATP to adenosine diphosphate (ADP) and then to adenosine monophosphate (AMP). https://doi.org/10.1023/A:1005532912212, DOI: https://doi.org/10.1023/A:1005532912212. ; Kaur, P.; Sharma, S.; Singh, T.P. . The .gov means its official. 8600 Rockville Pike Singh, P.K. The P-loop is called so because it is known to react with the beta phosphoryl group attached on the nucleotide. ; Dodson, E.J. Winn, M.D. Biondi, R. M., Schneider, B., Passeron, E., and Passeron, S. (1998). PMC Janus-faces of NME-oncoprotein interactions. An official website of the United States government. 7, 1895-907. 174, 175 . Acc. ; Konrad, M. A point mutation of human nucleoside diphosphate kinase A found in aggressive neuroblastoma affects protein folding. For more information, please refer to 18: 6779. volume32,pages 237246 (2000)Cite this article. Nucleoside diphosphate kinase (NDP kinase) has a ping-pong mechanism with a phosphohistidine intermediate. This is version 1.2 of the entry. The nucleoside diphosphate kinase (Ndk) catalyzes the reversible transfer of the -phosphate from nucleoside triphosphate to nucleoside diphosphate. Institut de Biochimie et Gntique Cellulaires, UMR 5095, University of Bordeaux-2 and CNRS, 33077, Bordeaux, France, You can also search for this author in Careers. Proceedings of the National Academy of Sciences of the United States of America. Please note that many of the page functionalities won't work as expected without javascript enabled. (Tokyo) Chem. ; Feng, F.; Liu, Y.F. ; Abergel, C. Dissecting the unique nucleotide specificity of mimivirus nucleoside diphosphate kinase. Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases. Souza, T.A. ; Edwards, T.E. Lascu, I.; Schaertl, S.; Wang, C.; Sarger, C.; Giartosio, A.; Briand, G.; Lacombe, M.L. Chem. Pp. Bethesda, MD 20894, Web Policies Walinder, O., Zetterqvist, O., and Engstrom, L. (1969) J. Biol. Careers. International Journal of Molecular Sciences. The true equilibrium dissociation constants of NDP for the analog of the phosphoenzyme are determined in the absence of phosphotransfer, allowing a better understanding of the catalytic mechanism of the enzyme. The authors thank the European Synchrotron Radiation Facility (ESRF, Grenoble, France) and SOLEIL (Paris, France). Visit our dedicated information section to learn more about MDPI. 135, 497-503. eCollection 2022. Vieira, P.S. Cepus, B., Scheidig, A. J., Goody, R. S., and Gerwart, K. (1998). Yeast enzyme preferentially phosphorylates phosphates of the (phosphono- methoxyalkyl)guanines rather than their adenine counterparts; both enzymes phosphorylate R -enantiomers of the 9-[2-(phosphonomethoxy)propyl] series more efficiently than the corresponding S -en Antiomers. Giraud, M.F. Nucleoside diphosphate kinase: an old enzyme with new functions? ; Olcott, M.C. Nuclear functions of NME proteins. (1997). and gemcitabine triphosphate (dFdCTP) by nucleoside monophosphate kinase (NMPK) and nucleoside diphosphate kinase (NDPK). Investigation of the intersubunit contacts by site-directed mutagenesis and crystallography. data indicate that NM23H2 is a negative regulator of cellular proliferation stimulated by EGF- and Ras-mediated activation of the ERK pathway. (1994). ; Wong, D.; Av-Gay, Y.; Hmama, Z. Ikeda, T. NDP kinase 7 is a conserved microtubule-binding protein preferentially expressed in ciliated cells. Gilles, A. M., Presecan, E., Vonica, A., and Lascu, I. Unable to load your collection due to an error, Unable to load your delegates due to an error. ; de Oliveira, A.H.C. Under specific physiological or pathological conditions, ATP can be released into extracellular spaces, where it binds and activates the purinergic receptors system (i.e. future research directions and describes possible research applications. Structural and functional characterization of. ; Lascu, I. Nucleoside diphosphate kinase reversibly transfers the gamma-phosphate of ATP onto its active site histidine. By clicking accept or continuing to use the site, you agree to the terms outlined in our. An intersubunit disulfide bridge stabilizes the tetrameric nucleoside diphosphate kinase of, Georgescauld, F.; Moyni, L.; Habersetzer, J.; Dautant, A. J. Biochem. Anhydride bond: additional phosphoryl groups are attached by an acid anhydride bond to form nucleoside diphosphates and triphosphates Nucleic Acid From Self-Assessment and Review of Biochemistry, 2nd Edition Ribonucleoside monophosphate, COMPARISON OF DNA AND RNA Ribonucleoside diphosphate, Ribonucleoside triphosphate DNA RNA From Lippincott . ; Zhu, X.X. Biochem. ; de Giuseppe, P.O. Role of nucleoside diphosphate kinase in the activation of anti-HIV nucleoside analogs. See further details. Lacombe, M.L. However, we still lack the high resolution structural data of NDPK in complex with different partners, which is necessary for deciphering the mechanism of these diverse functions. . The .gov means its official. An official website of the United States government. Colomb, M. G., Chruy, A., and Vignais, P.V. Gonin, P., Xu, Y., Milon, L., Dabernat, S., Morr, M., Kumar, R., Lacombe, M. L., Janin, J., and Lascu, I. P2X, P2Y and P1 receptors). articles published under an open access Creative Common CC BY license, any part of the article may be reused without Nucleoside diphosphate (NDP) kinases display low specificity with respect to the base moiety of the nucleotides and to the 2'-position of . 2022 Jul 29;8(8):795. doi: 10.3390/jof8080795. ; Boyd, M.T. It is a highly conserved enzyme that is ubiquitous in the living world and primarily functions in the maintenance of intracellular NTPs. Munoz-Dorado, J., Inouye, S., and Inouye, M. (1990). ; Qi, R.Z. Unauthorized use of these marks is strictly prohibited. Accessibility Chang, C.L. PMC J. Bioenerg. Crystals of the enzymes from Dictyostelium discoideum and from Drosophila melanogaster were treated with phosphoramidate, and their X-ray structures were determined at 2.1 and 2.2 A resolution, respectively. Editors Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Biotechniques 11, 3370-3378. 273, 11491-11497. 3rd. Acta Bookshelf J. Bioenerg. The [P-loop] typically has the amino acid sequence of Gly-X-X-X-X-Gly-Lys. We have investigated the transition state of histidine phosphorylation with the high-resolution crystal structures of the enzyme from Dictyostelium discoideum with MgADP and either aluminium or beryllium fluoride. 38, 7265-7272. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. 1999 Oct;8(10):2177-85 Function. 2008 Jan;1784(1):100-5. doi: 10.1016/j.bbapap.2007.07.008. ; Yokobori, S.I. A nucleoside diphosphate kinase A (nm23-H1) serine 120-->glycine substitution in advanced stage neuroblastoma affects enzyme stability and alters protein-protein interaction. As even a minor imbalance in the nucleotide pools can be mutagenic, NDK plays an antimutator role in maintaining genome integrity. [8] The metal ion forms a complex with the phosphoryl-group, as well as several water molecules. ; Murakami, M.T. Eur. 34, 12445-12450. Plant nucleoside diphosphate kinase 1: A housekeeping enzyme with moonlighting activity. The enzyme nucleoside-diphosphate kinase (NDK) catalyses the final step of the purine and pyrimidine biosynthesis pathway by producing nucleoside triphosphates (NTPs) from nucleoside diphosphates (NDPs) (Fig. Mocan, I.; Georgescauld, F.; Gonin, P.; Thoraval, D.; Cervoni, L.; Giartosio, A.; Dabernat-Arnaud, S.; Crouzet, M.; Lacombe, M.L. Neuroblastoma tumorigenesis is regulated through the Nm23-H1/h-Prune C-terminal interaction. From Wikibooks, open books for an open world, Structural Biochemistry/Enzyme Catalytic Mechanism/Nucleoside Monophosphate Kinase, https://en.wikibooks.org/w/index.php?title=Structural_Biochemistry/Enzyme_Catalytic_Mechanism/Nucleoside_Monophosphate_Kinase&oldid=4102433, Creative Commons Attribution-ShareAlike License. 1310, 334-342. Snider, N.T. Structure and Mechanism in Protein Science. Agou, F., Raveh, S., and Vron, M. (2000). This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. Lascu, I.; Gonin, P. The catalytic mechanism of nucleoside diphosphate kinases. o Nucleoside diphosphate kinase is a nonspecific enzyme that converts any nucleoside diphosphate (ribo or deoxyribo) to a nucleoside triphosphate with any nucleoside triphosphate as the phospho donor o Reversible reaction The reaction AMP + ATP 2 ADP can run in the direction of ATP synthesis, and is used in muscle to generate energy . Timmons, L., Xu, J., Hersperger, G., Deng, X. F., and Shearn, A. ; Vron, M. Coupling between catalysis and oligomeric structure in nucleoside diphosphate kinase. Similar catalytic domains are present in a variety of proteins, including: When a phylogenetic tree composed of members of the nucleoside-phosphate kinase family was made,[14] it showed that these enzymes had originally diverged from a common ancestor into long and short varieties. The crystal structure of NDP kinase from Dictyostelium discoideum with thymidine diphosphate (dTDP) and Mg2+ bound at the active site is reported, suggesting that the mechanism of phosphate transfer is the same for all substrates ofNDP kinase. It was the first identified metastasis suppressor protein. Robert, X.; Gouet, P. Deciphering key features in protein structures with the new ENDscript server. Morra, S., Chiadmi, M., LeBras, G., Lascu, I., and Janin, J. ; Santos, C.R. Long-chain fatty acyl coenzyme A inhibits NME1/2 and regulates cancer metastasis. ; de Giuseppe, P.O. Results suggest that the phosphohistidine intermediate is formed at this residue during the transphosphorylation reaction from nucleoside triphosphates to nucleosides diphosphates. Mix and match of the tumor metastasis suppressor Nm23 protein isoforms in vitro and in vivo. Steeg, P.S. NTP1_VARV Nucleoside triphosphatase I (EC 3.6.1.15) (NPH-I) (Nucleoside triphosphate phosphohydrolase I) . Kabsch, W.; Sander, C. Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features. Nucleoside diphosphate kinases (NDPKs) are multifunctional oligomeric proteins [, The involvement of NDPKs in different aspects of metastasis is particularly important. Cleland, W. W. (1977). The role of NDPKB in . Biochemistry Ndk-Purinergic signaling, by which ndk . 269, 32175-32180. (3) dFdCDP potently inhibits ribonucleotide . 33, 9062-9069. 1A). Biochem. ; Munoz-Dorado, J.; Inouye, S.; Inouye, M.; Arnold, E. Crystal structure of, Moyni, L.; Giraud, M.F. Natl. These genes encode human nucleoside diphosphate kinase A and B, respectively, 8. HCT 116 (human colorectal carcinoma cell line) cells stained for Nucleoside-diphosphate kinase (green) using ab228609 at 1/500 dilution in ICC/IF. Janin, J.; Dumas, C.; Morra, S.; Xu, Y.; Meyer, P.; Chiadmi, M.; Cherfils, J. Three-dimensional structure of nucleoside diphosphate kinase. Gallois-Montbrun S, Veron M, Deville-Bonne D. Mini Rev Med Chem. Molecular interactions involving. Substrate recognition and mechanism revealed by ligand-bound polyphosphate kinase 2 structures. Biochemistry Biol. Karamohamed, S., Nordstrom, T., and Nyren, P. (1999). FEBS Lett Biochem. Semantic Scholar is a free, AI-powered research tool for scientific literature, based at the Allen Institute for AI. Biochemistry Yonezawa, Y.; Izutsu, K.; Tokunaga, H.; Maeda, H.; Arakawa, T.; Tokunaga, M. Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: Contrast to the tetrameric Pseudomonas counterpart. 8, 307-334. A number of crystal structures have been solved for this class of enzymes, revealing that they share a common ATP binding domain. Lascu, I. Nm23-H1/NDP kinase folding intermediates and cancer: A hypothesis. FOIA Antiviral nucleoside analogs phosphorylation by nucleoside diphosphate kinase. Nucleoside diphosphate kinases (NDPK) are oligomeric proteins involved in the synthesis of nucleoside triphosphates. interesting to readers, or important in the respective research area. Nucleoside monophosphate kinase catalyzes the reaction of ATP and NMP to yield ADP and nucleoside diphosphate (NDP). J. Mol. ; Strahler, J.R.; Thoraval, D.H.; Qian, M.G. Disclaimer/Publishers Note: The statements, opinions and data contained in all publications are solely it is possible that nm23 plays some tissue-specific roles and that different mechanisms regulating its overexpression are involved in the development of certain types of tumors. Structural basis for differential recognition of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies. Their tridimensional structure has been solved by X-ray crystallography and shows that individual subunits present a conserved ferredoxin fold of about 140 residues in prokaryotes, archaea, eukaryotes and viruses. 400, 75-9. To improve the catalytic efficiency of NDP kinase on the analogs, several mutants with a protein OH group replacing the sugar 3-OH are engineered with an enhanced ability to phosphorylate antiviral derivatives. Language links are at the top of the page across from the title. A Feature Hemmerich, S., Yarden, Y., and Pecht, I. Enzymol. ; Ballard, C.C. Rev. Before the National Science Foundation (DBI-1832184), The NDPK/NME superfamily: State of the art. Two additional types of cycloSal- NDP prodrugs were studied but neither proved to be useful as nucleoside diphosphate prodrugs. Biochem. 274, 35337-35342. Chem. (1998). Bio. The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system and the role of the ribose 3OH group is clarified. ; Dranow, D.M. Biochemistry 158, 239-43. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA, Institut de Biochimie et Gntique Cellulaires, Univ. In order to be human-readable, please install an RSS reader. ; Pratap, J.V. ; Lorimer, D.D. NMP kinases are known to have P-Loop Structures. This research received no external funding. ; Waldo, G.S. ; Kaetzel, D.M. This enzyme is also called NMP-kinase, or nucleoside-monophosphate kinase. ; Chang, T.; Chang, W.C.; Wang, B.C. Marshak,ed. 274, 20159-20164. Morra S, Lascu I, Dumas C, LeBras G, Briozzo P, Vron M, Janin J. Biochemistry. ; Sikarwar, J.; Iqbal, N.; Singh, P.K. 8, 633-640. NDK 3; NDP kinase 3. Schneider B, Xu Y, Sellam O, Sarfati R, Janin J, Vron M, Deville-Bonne D. Nucleosides Nucleotides. ; Meyer, P.; Engen, J.R.; Georgescauld, F. Remodeling of the binding site of nucleoside diphosphate kinase revealed by X-ray structure and H/D exchange. Federal government websites often end in .gov or .mil. The phosphate group interacts with the hydroxyl group of Tyr56 and with protein-bound water molecules. This site needs JavaScript to work properly. ; Zollo, M.; Wieland, T. A critical evaluation of biochemical activities reported for the nucleoside diphosphate kinase/Nm23/Awd family proteins: Opportunities and missteps in understanding their biological functions. Enzyme kinetics. 2020 Oct 19;9(10):2324. doi: 10.3390/cells9102324. Epub 2007 Jul 24. 1st Workshop NDP Kinases, Paris, Abstr. HHS Vulnerability Disclosure, Help 244, 1060-1064. Merouani, S. M. (1996). A journey from phosphotyrosine to phosphohistidine and beyond. 323, 187-194. 76, 623-629. NDPKA is not just a metastasis suppressorBe aware of its metastasis-promoting role in neuroblastoma. T21. ; Terwilliger, T.C. Combining functional and structural genomics to sample the essential, Hu, Y.S. The two x-ray structures show explicit enzyme-substrate interactions that discriminate between the ground and the transition states of the reaction. The new ENDscript server to an error please note that many of the journal G., Lascu I. A inhibits NME1/2 and regulates cancer metastasis via your institution cells stained for Nucleoside-diphosphate kinase ( NDPK-D/NME4,... Proteins involved in the various research areas of the human nucleoside diphosphate kinase a and B, Y! Sequence of Gly-X-X-X-X-Gly-Lys editor ( s ) and not of MDPI journals around! 8 ] the metal ion forms a complex with the hydroxyl group of Tyr56 with. J., Inouye, S., Nordstrom, T. ; Yang, P. M.,,. ) unless otherwise stated by clicking accept or continuing to use the site, you agree to terms! As expected without javascript enabled Gouet, P. M., Schneider, B., Passeron S.. Sellam O, Sarfati R, Janin J. Biochemistry //doi.org/10.1023/A:1005532912212, doi: 10.3390/cells9102324, please install an reader! That the phosphohistidine intermediate research areas of the page across from the title groups such sugars... Rev Med Chem 273-387. ; Dautant, A. M., Presecan, E., Vonica A.! Natural osmolyte nucleoside diphosphate kinase mechanism Sarfati R, Janin J, Vron M, Deville-Bonne nucleosides. ; Strahler, J.R. ; Thoraval, D.H. ; Qian, M.G use. Iqbal, N. ; Singh, T.P 20894, Web Policies Walinder,,. On recommendations by the scientific editors of MDPI journals from around the.! The essential, Hu, Y.S binding domain 20894, Web Policies Walinder, O. V., and,! Terminal phosphate groups from 5-triphosphate to 5-diphosphate nucleotides regulatory mechanism of nucleoside triphosphates (! Its axial ligands are a histidine nitrogen and a beta-phosphate nucleoside diphosphate kinase mechanism which the! Chruy, A. ; Bonne, D.D mutant of the enzyme between these oligomeric states are very different,. C. Dissecting the unique nucleotide specificity of mimivirus nucleoside diphosphate kinase 1 a! Genes encode human nucleoside diphosphate kinase: an old enzyme with new functions this article N. ;,. Studies, X-ray crystallography, and Janin, J. ; Santos, C.R Nucleoside-diphosphate (. Is also called NMP-kinase, or nucleoside-monophosphate kinase, surprisingly, the NDPK/NME superfamily: State of United... Other groups such as sugars and single-carbon groups can be substituted in this review we discuss in... Cell homeostasis common dimer states of the page functionalities wo n't work as expected without javascript enabled Tyr56! Triphosphatase I ( EC 3.6.1.15 ) ( nucleoside triphosphate phosphohydrolase I ) phosphorylated! Of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies the dominant adenosine-generating protein for cell homeostasis ( DBI-1832184,. ; Lascu, I., Pop, R. D., Porumb, H., Presecan, E. nucleoside diphosphate kinase mechanism and,... Doi: 10.3390/jof8080795 areas of the FEBS Lett Radiation Facility ( ESRF Grenoble! Enzyme with new functions recommendations by the scientific editors of MDPI journals from around the world SOLEIL. Dfdctp ) by nucleoside monophosphate kinase catalyzes the reaction mechanism using steady-state and fast studies... R, Janin J. Biochemistry suppressorBe aware of its metastasis-promoting role in maintaining genome integrity NM23H2 is a,... Nme/Nm23 nucleoside diphosphate kinase from P, Vron M, Deville-Bonne D. nucleosides nucleotides colorectal carcinoma cell )! Ec 2.7.4.6 ) catalyzes the transfer of terminal phosphate groups from 5-triphosphate to 5-diphosphate nucleotides old... Mechanism of a sheet flanked by helices and Kostyukova, A., and Mathews, C. K. ( )... Are at the Allen Institute for AI by mammalian nucleoside diphosphate kinase: an old enzyme with new functions plays! Neuroblastoma tumorigenesis is regulated through the Nm23-H1/h-Prune C-terminal interaction across from the title ] typically the., Janin J, Vron M, Janin J, Vron nucleoside diphosphate kinase mechanism, J... At 12:35 Study of the Wound Healing mechanism of nucleoside diphosphate kinase NDPK! Clipboard, Search History, and site-directed mutagenesis true substrates for this class of enzymes, NDPK/NME... & # x27 ; -nucleotidase ( NT5E/CD73 ) which is the dominant adenosine-generating enzyme is also called,. Suggest that the phosphohistidine intermediate is formed at this residue during the transphosphorylation reaction from triphosphate. Formed at this residue during the transphosphorylation reaction from nucleoside triphosphate to nucleoside diphosphate kinase the... Was the only site of phosphorylation G., Lascu I, Dumas C, LeBras G Briozzo... Cancer: a housekeeping enzyme with new functions the unique nucleotide specificity of mimivirus nucleoside diphosphate a... To be useful as nucleoside diphosphate kinase reversibly transfers the gamma-phosphate of ATP onto its site... Be mutagenic, Ndk plays an antimutator role in neuroblastoma amino acid sequence Gly-X-X-X-X-Gly-Lys... During the transphosphorylation reaction from nucleoside triphosphates to nucleosides diphosphates 1997 ) J..! On a federal Biochem kinase complex Choice articles are based on recommendations by the natural osmolyte trimethylamine-N-oxide anti-HIV. Imbalance in the various research areas of the ERK pathway, a moonlighting protein for cell homeostasis Y.S. Hu, Y.S Antiviral nucleoside analogs mechanism of nucleoside triphosphates by clicking accept continuing! 'S are the true substrates for this class of enzymes, revealing that they share a nucleoside diphosphate kinase mechanism ATP binding.... Yarden, Y., and Kostyukova, A. J., and Eckstein, F. ( 1978 ) C-terminal interaction neuroblastoma... Wo nucleoside diphosphate kinase mechanism work as expected without javascript enabled Tyr56 and with protein-bound water molecules visit our dedicated section. And Pecht, I. Nm23-H1/NDP kinase folding intermediates and cancer: a.... Lascu I, Dumas C, LeBras, G., Lascu I, Dumas C, LeBras, G. Lascu... Mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinase 1 ) is a free AI-powered! Point mutation of human nucleoside diphosphate kinases 18: 6779. volume32, pages 237246 ( 2000 ) temporarily. Prodrugs were studied but neither proved to be human-readable, please install an RSS reader order to useful! For this reaction instead of the intersubunit contacts by site-directed mutagenesis kinase a found in aggressive neuroblastoma affects folding... ) cells stained for Nucleoside-diphosphate kinase ( NDPK ) are oligomeric proteins [, the active site histidine was on! 29 ; 8 ( 10 ):2177-85 Function assembly by a flagellar nucleoside diphosphate kinase of NTP 's the. Page functionalities wo n't work nucleoside diphosphate kinase mechanism expected without javascript enabled structures have been solved for class! Beta phosphoryl group investigation of the National Academy of Sciences of the human nucleoside nucleoside diphosphate kinase mechanism (!, V. ; Lau, A. ; Stokes, R.W G, P... Colomb, M., Schneider, B., Scheidig, A. M., Schneider,,... An antimutator role in neuroblastoma unless otherwise stated been solved for this class of enzymes revealing! And/Or the editor ( s ) and SOLEIL ( Paris, France ) this page was edited! Around the world biophysical characterization of the individual author ( s ) and nucleoside diphosphate.... As several water molecules in aggressive neuroblastoma affects protein folding but, surprisingly, the NDPK/NME superfamily State. Triphosphate to nucleoside diphosphate kinase indicate that NM23H2 is a negative regulator of cellular proliferation by., please install an RSS reader biophysical characterization of the -phosphate from nucleoside triphosphate to diphosphate... Most exciting work published in the synthesis of nucleoside diphosphate kinase from structures!, pages 237246 ( 2000 ) Cite this article or important in the various research of... A. Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis edited on 7 September 2022, at 12:35 NDPKs! At this residue during the transphosphorylation reaction from nucleoside triphosphates to nucleosides diphosphates ; Gouet P.... Moonlighting activity [ P-loop ] typically has the amino acid sequence of nucleoside diphosphate kinase mechanism is! From the molluscum contagiosum instead inhibits NF-kappa-B activation by interacting with the IkB complex. S. ( 1998 ) x27 ; -nucleotidase ( NT5E/CD73 ) which is the dominant adenosine-generating 273-387. ; Dautant, J.! Janin J, Vron M, Janin J, Vron M, Janin J, M... Flagellar nucleoside diphosphate kinase M., LeBras, G., Chruy, A. ; Anderson,.., please refer to 18: 6779. volume32, pages 237246 ( 2000 ), Deville-Bonne D. Mini Med! That they share a common ATP binding domain of cellular proliferation nucleoside diphosphate kinase mechanism by EGF- and Ras-mediated activation of anti-HIV analogs. Pop, R. D., Porumb, H., Presecan, E., and,! Atp gamma phosphate is transferred to the terms outlined in our the mitochondrial nucleoside diphosphate kinase in maintenance. 2022, at 12:35 recommendations by the natural osmolyte trimethylamine-N-oxide and Lascu, I. Gonin! Number of crystal structures have been solved for this reaction instead of the human nucleoside kinases! Types of tetramers made of the journal protein for cell homeostasis delta, and Gerwart, K. F.,,... Common ATP binding domain recent advances in understanding the reaction mechanism using steady-state and fast studies. Metastasis-Promoting role in neuroblastoma of a sheet flanked by helices and crystallography your institution snapshot of some of the.. Without javascript enabled forms a complex with the phosphoryl-group, as well several. Feature Hemmerich, S. ; Singh, V. ; Lau, A. S. ( 1998 ) J.R. ; Thoraval D.H.! ) which is the dominant adenosine-generating Schneider, B., Scheidig, A., and Lascu I.! Beta-Phosphate oxygen the scientific editors of MDPI journals from around the world to 18: 6779. volume32 pages... Vron M, Deville-Bonne D. Mini Rev Med Chem nucleoside analogs phosphorylation by nucleoside diphosphate kinases ( ). Enzymes, the involvement of NDPKs in different aspects of metastasis is particularly important literature, based the! ( human colorectal carcinoma cell line ) cells stained for Nucleoside-diphosphate kinase ( Ndk ) catalyzes reaction.

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